★GentleMACS/单细胞悬液制备文献集锦

种属

处理组织

文章名称

human

Decidual tissues（蜕膜组织）

LILRB4 Decrease on uDCs Exacerbate Abnormal Pregnancy Outcomes Following Toxoplasma gondii Infection

human

Brain（脑组织）

Imp2 controls oxidative phosphorylation and is crucial for preserving glioblastoma cancer stem cells

human

breast cancer（乳腺癌）

DNA methyltransferase inhibition upregulates MHC-I to potentiate cytotoxic T lymphocyte responses in breast cancer

human

glioblastoma tumor（成胶质细胞瘤肿瘤）

Transcriptional control of O 6 -methylguanine DNA methyltransferase expression and temozolomide resistance in glioblastoma

human

Liver（肝脏组织）

Intrahepatic Vγ9Vδ2 T-cells from HCV-infected patients show an exhausted phenotype but can inhibit HCV replication

human

Lung（肺脏组织）

NKG2D Regulation of Lung Pathology and Dendritic Cell Function Following Respiratory Syncytial Virus Infection

human

lung cancer（肺癌组织）

①DNA methylation in small cell lung cancer defines distinct disease subtypes and correlates with high expression of EZH2

②Conversion to stem-cell state in response to microenvironmental cues is regulated by balance between epithelial and mesenchymal features in lung cancer cells

human

Mitochondria
Extraction Kit, tissue（组织中线粒体的提取）

①Efficient Isolation of Pure and Functional Mitochondria from Mouse Tissues Using Automated Tissue Disruption and Enrichment with Anti-TOM22 Magnetic Beads

②A comparison of mitochondrial DNA isolation methods in frozen post-mortem human brain tissue: applications for studies of mitochondrial genetics in brain disorders.

human

nasal mucosa（鼻粘膜组织）

IL-17A as a regulator of neutrophil survival in nasal polyp disease of patients with and without cystic fibrosis

human

Neural（神经组织）

①MIF receptor CD74 is restricted to microglia/macrophages, associated with a  M1-polarized immune milieu, and prolonged patient survival in gliomas

②Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers

③1,25-Dihydroxyvitamin D3 enhances neural stem cell proliferation and oligodendrocyte differentiation

human

Neurosphere（神经球）

①Integrated Analysis of miRNA and mRNA Expression inChildhood Medulloblastoma Compared with Neural Stem Cells

②Activated adult microglia influence retinal progenitor cell proliferation and differentiation toward recoverin-expressing neuron-like cells in a co-culture model

human

ovarian and renal carcinoma（卵巢癌和肾癌）

Mitochondrial morphological and functional reprogramming following CD137(4-1BB) costimulation

human

Skin, cartilage and fat tissue（皮肤，软骨和脂肪组织）

Perfusion-decellularization of human ear grafts enables ECM-based scaffolds for auricular vascularized composite tissue engineering

human

Tumor（肿瘤组织）

①Angiogenic factor-driven inflammation promotesextravasation of human proangiogenic monocytes to tumours

②Apoptotic Cancer Cells Suppress 5-Lipoxygenase in Tumor-Associated Macrophages

③In Vitro Efficient Expansion of Tumor Cells Deriving from Different Types of Human Tumor Samples

④Rapid nodal staging of head and neck cancer surgical specimens with flow cytometric analysis

mouse

Heart（心脏组织）

Revisiting Cardiac Cellular Composition

mouse

Lamina Propria（肠粘膜固有层组织）

①Heme oxygenase-1 deficiency promotes the development of necrotizing enterocolitis-like intestinal injury in a newborn mouse model

②Social stress-enhanced severity of Citrobacter rodentium-induced colitis is CCL2-dependent and attenuated by probiotic Lactobacillus reuteri

mouse

Lung（肺脏组织）

①A New Approach for the Study of Lung Smooth Muscle Phenotypes and Its Application in a Murine Model of Allergic Airway Inflammation

②Obesity-induced adipokine imbalance impairs mouse pulmonary vascular endothelial function and primes the lung for injury

③In situ vaccination with cowpea mosaic virus nanoparticles suppresses metastatic cancer

mouse

Lung/Lamina Propria（肺脏组织/肠粘膜固有层组织）

①The transcription factor Bcl11b is specifically expressed in group 2 innate lymphoid cells and is essential for their development

②Single-cell RNA-seq identifies a PD-1hi ILC progenitor and defines its development pathway

③The transcription factor Bcl11b is specifically expressed in group 2 innate lymphoid cells and is essential for their development

④ICOS regulates the pool of group 2 innate lymphoid cells under homeostatic and inflammatory conditions in mice

mouse

Tumor（肿瘤组织）

γ-Secretase inhibitor reduces immunosuppressive cells and enhances tumour immunity in head and neck squamous cell carcinoma

mouse

ear/skin（皮肤组织）

Requirement for CD28 in Effector Regulatory T Cell Differentiation, CCR6 Induction, and Skin Homing

mouse

Epidermis（表皮组织）

①Tissue-resident macrophages originate from yolk-sac-derived erythro-myeloid progenitors

②Mice deficient in heparanase exhibit impaired dendritic cell migration and reduced airway inflammation

mouse

Lamina Propria（肠粘膜固有层组织）

①a context-Dependent role for αv integrins in regulatory T cell accumulation at sites of inflammation

②Environmental-mediated intestinal homeostasis in neonatal mice

③Direct interaction between cholera toxin and dendritic cells is required for oral adjuvant activity

④Increased dietary vitamin D suppresses MAPK signaling, colitis, and  colon cancer

mouse

Lung（肺脏组织）

①Growth of Mycobacterium tuberculosis in vivo segregates with host macrophage metabolism and ontogeny

②Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator

③Miro1 regulates intercellular mitochondrial transport & enhances mesenchymal stem cell rescue efficacy

mouse

Lung cancer/MDSC分选（肺癌组织）

Inhibition of curcumin on myeloid-derived suppressor cells is requisite for controlling lung cancer

mouse

lung/adipose（肺脏组织/脂肪组织）

High-Resolution PET Imaging with Therapeutic Antibody-based PD-1/PD-L1 Checkpoint Tracers

mouse

lung/tumor（肺脏组织/肿瘤组织）

A Wnt-producing niche drives proliferative potential and progression in lung adenocarcinoma

mouse

Muscle（肌肉组织）

①Dystrophin expression in muscle stem cells regulates their polarity and asymmetric division

②Omigapil Ameliorates the Pathology of Muscle Dystrophy Caused by Laminin- 2 Deficiency

mouse

neonatal（新生鼠心脏组织）

①Transgenic systems for unequivocal identification of cardiac myocyte nuclei and analysis of cardiomyocyte cell cycle status

②Novel Roles of GATA4/6 in the Postnatal Heart Identified through Temporally Controlled, Cardiomyocyte-Specific Gene Inactivation by Adeno-Associated Virus Delivery of Cre Recombinase

mice

neonatal dissociation kit/Neonatal Cardiomyocyte
Isolation kit（新生鼠心脏组织）

The extracellular matrix protein agrin promotes heart regeneration in mice

mouse

Neural（神经组织）

①Assessment of Adult Neurogenesis in Mice

②Isolation of multipotent neural stem or  progenitor cells from both the dentate gyrus and subventricular zone of a single adult mouse

mouse

neural（CNS）（中枢神经组织）

Isolation of Vascular Endothelial Cells from Intact and Injured Murine Brain Cortex—Technical Issues and Pitfalls in FACS Analysis of the Nervous Tissue

mouse

Spleen（脾脏组织）

①Killed but metabolically active Pseudomonas aeruginosa-based vaccine induces protective humoral- and cell-mediated immunity against Pseudomonas aeruginosa pulmonary infections

②Systemic immune-checkpoint blockade with anti-PD1 antibodies does not alter cerebral amyloid-b burden in several amyloid transgenic mouse models

mouse

Tumor（肿瘤组织）

①Potentiating tumor immunity using aptamer-targeted RNAi to render CD8 + T cells resistant to TGFβ inhibition

②Therapeutic Impact of Nanoparticle Therapy Targeting Tumor Associate Macrophages

③Macrophage-dependent nitric oxide expression regulates tumor cell detachment and metastasis after IL-2/anti-CD40 immunotherapy

rat

neonatal （新生儿心脏组织）

Reduced matrix rigidity promotes neonatal cardiomyocyte dedifferentiation, proliferation and clonal expansion

rat

Neural（神经组织）

 Flow cytometry for receptor analysis from ex-vivo brain tissue in adult rat

lion

Eye（视网膜组织）

Role of lysophosphatidic acid in the retinal pigment epithelium and photoreceptors